Super-Resolution from a Single Frame
Published:
Standard SOFI needs hundreds of frames of blinking emitters to beat the diffraction limit. You acquire a long temporal stack, compute cumulants across time, and the nonlinear statistics sharpen the point spread function beyond what any single exposure can resolve. It works beautifully — but it demands fluorophores that blink stochastically, and it demands patience while you collect enough frames for the statistics to converge.
